LOCUS       pDONR™/Zeo
DISCLAIMER  Certain terms are trademarks or registered trademarks of Invitrogen 
            Corporation. See "Intellectual Property" in the Help file for more 
            information.
FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1577..1673)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1688..1707)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1715..1731
                     /note="M13 Reverse priming site"
     gene            1844..2653
                     /note="Kanamycin resistance gene"
     rep_origin      2774..3447
                     /note="pUC origin"
     vector          join(1585..3450,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1570
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="MGC:199245 IMAGE:100069234"
                     /clone_lib="NIH_MGC_428"
                     /lab_host="DH10B"
                     /focus
                     /note="Vector: pENTR223.1 with stop codon"
     source          692..1570
                     /organism="Homo sapiens"
                     /mol_type="other DNA"
                     /db_xref="taxon:9606"
     gene            692..1570
                     /gene="HS3ST6"
                     /db_xref="GeneID:64711"
                     /db_xref="HGNC:14178"
     CDS             692..1570
                     /dnas_title="heparan sulfate (glucosamine)
                     3-O-sulfotransferase 6"
                     /gene="HS3ST6"
                     /codon_start=1
                     /product="heparan sulfate (glucosamine)
                     3-O-sulfotransferase 6"
                     /protein_id="AAI72540.1"
                     /db_xref="GI:225000902"
                     /db_xref="GeneID:64711"
                     /db_xref="HGNC:14178"
                     /translation="MLLVALVLGAYCLCALPGRCPPAARAPAPAPAPSEPSSSVHRPG
                     APGLPLASGPGRRRFPQALIVGVKKGGTRALLEFLRLHPDVRALGSEPHFFDRCYERG
                     LAWYRSLMPRTLDGQITMEKTPSYFVTREAPRRIHAMSPDTKLIVVVRNPVTRAISDY
                     AQTLSKTPGLPSFRALAFRHGLGPVDTAWSAVRIGLYAQHLDHWLRYFPLSHFLFVSG
                     ERLVSDPAGEVGRVQDFLGLKRVVTDKHFYFNATKGFPCLKKAQGGSRPRCLGKSKGR
                     PHPRVPQALVRRLQEFYRPFNRRFYQMTGQDFGWG"
     misc_feature    692..1570
                     /note="HS3ST6 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccgctgcccg ccggccgccc gcgcccccgc
        721 gccggccccc gcgccctccg agccgtccag ctccgtccac cgcccgggag cacccggcct
        781 gcctttggcc agcggtcccg gccgccggcg cttcccgcaa gcgctcatcg ttggcgtgaa
        841 gaagggcggc acgcgcgccc tgctggagtt tctgcggctg caccccgacg tccgcgcgct
        901 gggctctgag ccccacttct tcgacaggtg ctacgagcgc ggcctcgcct ggtaccggag
        961 tctgatgccc cgaaccctgg atgggcagat caccatggag aagaccccca gctacttcgt
       1021 gacgcgagag gccccccgcc gcatccacgc catgtccccg gacacgaagc tgatcgtggt
       1081 ggtgcggaac cccgtgaccc gggccatctc cgactacgcc cagacgctct ccaagacccc
       1141 gggcctgccc agcttccgcg ccctggcctt ccgccacggc ctgggccccg tggacacagc
       1201 ctggagcgcc gtccgcatcg gcctgtacgc ccagcacctg gaccactggc tgcgctactt
       1261 ccccctgtcc cacttcctgt tcgtcagcgg ggagcgtctg gtcagcgacc cggccggaga
       1321 ggtcggccgc gtgcaggact tcctgggcct gaaacgggtc gtcacggaca agcacttcta
       1381 cttcaacgcc accaagggct tcccctgcct caagaaggcc cagggcggca gccgtccccg
       1441 ctgcctgggc aagtccaagg gccggccaca cccacgcgtg ccccaggccc tggtccggcg
       1501 cctgcaggag ttctaccggc ccttcaaccg caggttctac cagatgacgg gccaggactt
       1561 cggctggggc TAGGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT
       1621 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC
       1681 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG
       1741 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC
       1801 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG
       1861 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG
       1921 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA
       1981 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA
       2041 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT
       2101 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA
       2161 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT
       2221 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG
       2281 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA
       2341 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT
       2401 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA
       2461 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA
       2521 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT
       2581 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA
       2641 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC
       2701 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA
       2761 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
       2821 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
       2881 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
       2941 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
       3001 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
       3061 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
       3121 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
       3181 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
       3241 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
       3301 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
       3361 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
       3421 GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//